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THOMSON BIOAdvantageÖ Columns

High Performance Columns and Guard Systems

C18, C8 & C4 (100, 200 & 300 Angstrom) for peptide, protein and biomolecule analysis.

Unique Base Deactivated columns. Exceptional separations for amines and polar molecules.

300┼ or 200┼ for Peptides and Proteins.

Available in stainless steel or PEEK.á Designed to use the Direct Connect Guard System.

Thomson reverse phase columns are designed for the chromatography of peptides and proteins. They are characterized by high resolution, efficiency and peak symmetry.

Available in C4, C8 or C18 phases, 100┼, 200┼, or 300┼ pore sizes and 3Ám or 5Ám particle sizes.

Take advantage of the better resolution, for small molecules, afforded by the high surface area, 100┼ packing, or separate larger molecules on the 300┼ packing. Their identical selectivity allows you to maintain the same elution order from one product to the other.

Chromatographing small proteins or peptides on a 300┼ packing is conventional wisdom. But in many cases the higher surface area support would be the better choice. Now we offer an intermediate surface area with our 200┼ materials.

These columns, perfect for LC/MS, provide excellent resolution without TFA or other modifers. Or chose a modifier other than TFA, to enhance selectivity for the compounds of interest.

Guard
Direct Connect Guard Columns

Base Deactivated Columns

Hodges Peptide Mix
Tryptic Digest of Transferrin
Parabens
Synthetic Oligonucleotide

BIOAdvantage Basic & BHS are unique materials ideally suited for chromatographing basic compounds. Compare the chromatogram of Pyridine & Phenol in 40% acetronitrile/water unmodified by TFA or other enhancing additive.

Other columns, thought to be the standard of the industry, make these compounds tail badly and require suitable modifiers to "shield" active sites on the solute or packing surface. BIOAdvantage provides distinctive peaks without additives.

BioAdvantage Pro for Peptides and Small Proteins

BioAdvantage Pro 200 & 300┼ wide pore HPLC columns are traditionally used for biomolecule analysis since the molecular radii of large peptides and proteins are better matched than with smaller pore size columns (80 - 120┼) typically used for small molecule analysis. 300┼ sorbents, however, have reduced surface area, thus less capacity and selectivity than would be had with smaller pore size materials.

Thomson's new BioAdvantage Pro200, 200┼ packing, presents a powerful alternative for the analysis of peptides of ~19kD and less.The chromatography of phosphopeptides (on the right) illustrates this principle. Selectivity is identical from one packing to the other.

Our 300┼ phases can operate in low pH conditions and be washed at high pH. These are just some of the BIOAdvantageÖ advantages.

Available in C4 and C18 phases, these columns are a perfect replacement for the discontinued Vydac« 218TP and 214TP columns.

Phosphopeptides
Phosphopeptides on BioAdvantage Pro200

Direct Connect Column Protection System

Temperature: 80░C maximum
Pressure: ~ 5000 psi
Variance: Approximately 20Ál2 at 200Ál/min. This means, under isocratic conditions, some performance loss will be experienced with column ID's less than 4.6 mm.
Solvents: Compatible with most solvents. DMSO, Me2Cl2 and THF cause PEEK to swell over time. So they are not recommended for routine use.
Operating Specifications

High performance analytical columns and cartridges require a protection device equally as efficient.

Direct Connect Guards are a highly efficient column protection device that guards the analytical column from both particulate matter and chemical contamination.

Its new, unique PEEK holder is precision manufactured to very tight tolerances and can be hand tightened to 5000 psi.

Direct connection to your HPLC column results in zero dead volume.

The holder is supplied with a tube stop depth compatible with most of today's HPLC fittings. If you need a different stop depth, it's a simple matter to change the connecting tube to accommodate your HPLC column's end fitting.

The small volumes and direct connection means performance loss due to our guard is minimal if even measurable.

They will not alter the selectivity of your analytical column.

Selected Applications

Ordering Information

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