Fibrinogen

Fibrinogen was analyzed using AFpak ALC-894 (an affinity column).


Sample : Fibrinogen (Sigma)

Column       : Shodex AFpak ALC-894 (8.0mmID*50mm)
Eluent       : (A); 0.05M Tris-HCl(pH7.4) + 0.15M NaCl + 0.5mM CaCl2 + 0.5mM MnCl2 
               (B); (A) + 0.1M Methyl mannoside
               Step gradient: (A) to (B)
Flow rate    : 0.5mL/min
Detector     : UV(280nm)
Column temp. : Room temp.