C-Reactive Protein

C-Reactive protein was analyzed using AFpak APE-894 (an affinity column).


Sample : C-Reactive protein (Sigma)

Column       : Shodex AFpak APE-894 (8.0mmID*50mm)
Eluent       : (A); Tris-HCl(pH7.0) + 0.15M NaCl + 1mM CaCl2 
               (B); (A) + 0.2M Phosphorylcholine
               Step gradient: (A) to (B) 
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : Room temp.