Effects of Temperature on GPC Clean-up

Two different temperature settings (room temerature and 40 deg-C) were compared for the GPC clean-up method using CLNpakEV-2000 AC. At room temperature, the high viscosity causes peak broadening and increases the analysis time. Therefore, analysis at 40 deg-C is preferred. In Food Sanitation Law #1003001 (Released by Ministry of Health, labour and welfare in Japan, 2006), analysis temperature is set at 40 deg-C.


Sample : 5mL
Fatty tuna
Acrinathrin 40mg/L
Tricyclazole 40mg/L

Column       : Shodex CLNpak EV-G AC (20.0mmID*100mm) + EV-2000 AC (20.0mmID*300mm)
Eluent       : Acetone/Cyclohexane=1/4
Flow rate    : 5mL/min
Detector     : UV(254nm)