Recovery of Proteins (KW-803)
Both PROTEIN
KW-803 and Company A column (a column for aqueous SEC (GFC)) show
high mass recoveries of ribonuclrease A and thyroglobulin. Such high recoveries
are attributed to minimum adsorption properties. Furthermore, the calibration
curve obtained by sample loading and peak areas shows good linearity in a wide
range between 1 micro-g to 100 micro-g.
| Sample |
Column |
1 micro-g |
5 micro-g |
10 micro-g |
50 micro-g |
100 micro-g |
| Ribonuclease A |
KW-803 |
108.6 |
96.3 |
98.2 |
98.8 |
99.0 |
| Company A |
110.5 |
100.6 |
97.7 |
98.3 |
98.9 |
| Thyroglobulin |
KW-803 |
106.1 |
98.2 |
97.3 |
98.7 |
98.6 |
| Company A |
111.9 |
92.6 |
93.9 |
95.9 |
96.6 |
Columns : Shodex PROTEIN KW-803 (8.0mmID*300mm), Company A column
Eluent : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : Room temp.
