Standard Proteins (25) (KW403-4F)

Ten kinds of protein standards were analyzed using KW403-4F (a semi-micro column for aqueous SEC (GFC)).


Sample : 5micro-L
1. Thyroglobulin (MW: 669,000)
2. gamma-Globulin (MW: 158,000)
3. BSA (MW: 67,000)
4. Ovalbumin (MW: 43,000)
5. Peroxidase (MW: 40,200)
6. beta-Lactoglobulin (MW: 35,000)
7. Myoglobin (MW: 17,000)
8. Ribonuclease A (MW: 13,700)
9. Aprotinin (MW: 6,500)
10. Vitamin B₁₂ (MW: 1,350)

Column       : Shodex KW403-4F (4.6mmID*300mm)
Eluent       : 50mM Sodium phosphate buffer(pH7.0) + 0.3M NaCl
Flow rate    : 0.33mL/min
Detector     : UV(220nm) (small cell volume)
Column temp. : 30deg-C