Column : Shodex AFpak AGT-894 (8.0mmID*50mm) Eluent : (A); 22mM Phosphate buffer(pH7.0) (B); 50mM Tris buffer(pH9.6) + 5mM Glutathione Step gradient: (A) to (B) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : Room temp.