Adenosin Phosphates

Adenosin-5'-monophosphate, adenosin-5'-diphosphate and adenosin-5'-triphosphate were separated using AFpak AAF-894 (an affinity column) with 0.1M Ethylmorpholine-acetate (pH7.0) as the eluent.


Sample : Adenosin phosphate
1. Adenosin-5'-monophosphate, AMP
2. Adenosin-5'-diphosphate, ADP
3. Adenosine-5'-triphosphate, ATP

Column       : Shodex AFpak AAF-894 (8.0mmID*50mm)
Eluent       : 0.1M Ethylmorpholine-acetate buffer(pH7.0)
Flow rate    : 1.0mL/min
Detector     : UV(260nm)
Column temp. : Room temp.