The study of Injection Volume ; GS-320 Series Columns

The figure shows the effect of the injection volume to the separation for the analysis of uracil using GS-320 series columns.

(Recommended injection volume)

Product name	Size (mm) ID x L	Injection volume (micro-L)
GS320A-1E	1.0 x 250	1
GS320A-2E	2.0 x 250	10
GS-320 HQ	7.5 x 300	100

Sample : Uracil
2ng (GS320A-1E)
10ng (GS320A-2E)
200ng (GS-320 HQ)

Columns  : Shodex GS320A-1E (1.0mmID*250mm)
           Shodex GS320A-2E (2.0mmID*250mm)
           Shodex Asahipak GS-320 HQ (7.5mmID*300mm)
Eluent   : 10mM HCOONH4 buffer(pH4.0)
Detector : UV(260nm) cell volume; 3micro-L, optical path length; 7mm
Tubing   : 0.1mmID PEEK tube
Injector : Rheodyne 8125