Orange Juice

Saccharides in orange juice were analyzed using SUGAR SC1011 (a column for saccharides analysis). For the pretreatment of juice, first, the sample was filtrated 2 times using a 0.45 micron disposal filter. Then, it was treated with a mini-column packed with anion ion exchanger. And it was filtered again. In front of glucose and fructose peaks, a large peak was observed and it was considered to be oligosaccharides.


Sample : Mandarin orange juice, 5micro-L
1. Sucrose
2. Glucose
3. Fructose

Column       : Shodex SUGAR SC1011 (8.0mmID*300mm)
Eluent       : H2O
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 80deg-C