Orange and Melon

Saccharides in orange and melon were analyzed using SUGAR SH1011. Besides saccharides, some unknown peaks were observed though organic acids and pigments were eliminated by the mini-column.

(the pretreatment of fruit juice)
First, the sample was filtrated using a 25 micron disposable filter.
Then, it was treated with a mini-column packed with anion exchanger. And, it was filtrated again.


Sample :
Mandarin orange, 2.5micro-L
Musk melon, 2micro-L
1. Glucose
2. Fructose

Columns      : Shodex SUGAR SH-G (6.0mmID*50mm) + SH1011 (8.0mmID*300mm)
Eluent       : 5mM H2SO4 aq.
Flow rate    : 1.0mL/min
Detector     : Shodex RI, UV(210nm)
Column temp. : 50deg-C