Durability against Acidic Solvents

Since Asahipak NH2P-50 column is chemically stable, the durability against acidic solvents is better than that of silica-based amino columns.

Testing procedure
(1)Testing(63hrs)
 Eluent       : 0.01M H2SO4(pH2)
 Flow rate    : 0.1mL/min
 Column temp. : Room temp.
(2)Replacement(1hr)
 Eluent       : H2O
 Flow rate    : 0.5mL/min
 Column temp. : Room temp.
(3)Equilibrium(1hr)
 Eluent       : 0.1M NaOH
 Flow rate    : 0.5mL/min
 Column temp. : Room temp.


Comparison between before and after testing
SampleBefore testingAfter testing
Elution volume
(mL)
Plate number Elution volume
(mL)
Plate number
1.Fructose6.739600 6.708400
2.Glucose8.597900 8.588500
3.Sucrose11.889600 11.939300
4.Maltose14.248500 13.908600


Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30deg-C