Durability against Alkaline Solvents

Since the packing material of Asahipak NH2P-50 column is polymer, it is durable against alkaline solvents. To the contrary, silica-based amino columns are not durable against alkaline solvents.

Testing procedure 
(1)Equilibrium(1hr) Conditions before testing
 Eluent       : 100mM Ammonium acetate(pH9.3)
 Flow rate    : 0.1mL/min
 Column temp. : Room temp.
 
(2)Analysis
 
 
  

(3)Testing(160hrs)
 Eluent       : 5mM NaOH(pH11.4)/CH3CN=25/75
 Flow rate    : 0.1mL/min
 Column temp. : 30deg-C
 
(4)Replacement(0.5hr)
 Eluent       : H2O
 Flow rate    : 0.5mL/min
 Column temp. :  Room temp.
 
(5)Replacement(0.5hr)
 Eluent       : H2O
 Flow rate    : 0.5mL/min
 Column temp. :  Room temp.
 
(6)Analysis


Comparison between before and after testing
SampleBefore testingAfter testing
Elution volume
(mL)		Plate number Elution volume
(mL)		Plate number
1.Fructose7.527100 7.628100
2.Glucose9.636100 9.687700
3.Sucrose13.418400 13.409700
4.Maltose16.167200 16.087400


Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/H2O=75/25
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 30deg-C