Effect of Temperature (2)
Raffinose was separated using SUGAR SH1011
. Since raffinose can be easily hydrolyzed by acids, it should be analyzed at ordinary temperature.
The situation is the same for other saccharides such as sucrose.
Sample : Raffinose
Column : Shodex SUGAR SH1011 (8.0mmID*300mm)
Eluent : 0.005M H2SO4 aq.
Flow rate : 0.6mL/min
Detector : Shodex RI
Column temp. : (above); 60deg-C, (below); 25deg-C