Saccharides Analysis in Ionic Liquid (DC-613)

Mono- and di-saccharides in ionic liquid were analyzed by RSpak DC-613. Saccharides were separated under ligand-exchange and HILIC separation modes. The presence of one type of ionic liquids, 1-Ethyl-3-methylimidazolium chloride, did not interfere with saccharide separation.


Sample : 0.1% each, 10micro-L
1. Xylose
2. Sucrose
3. Cellobiose
4. Lactose
※Sample solution :
1% 1-Ethyl-3-methylimidazolium Chloride/CH₃CN=50/50

Column       : Shodex RSpak DC-613 (6.0mmID*150mm)
Eluent       : CH3CN/H2O=70/30
Flow rate    : 0.8mL/min
Detector     : Shodex RI
Column temp. : 50deg-C