N-Acetyl-Chitooligosaccharides

Chitooligosaccharides were analyzed using Asahipak NH2P-50 4E (a column for saccharides analysis).


Sample : 2% Mixture of N-Acetyl-chitooligosaccharides, 20micro-L
(Seikagaku Co. code:800104 Chitooligosaccharides Mixture)

1. N-Acetyl-D-glucosamine
2. di-N-Acetyl-chitobiose
3. tri-N-Acetyl-chitotriose
4. tetra-N-Acetyl-chitotetraose
5. penta-N-Acetyl-chitopentaose
6. hexa-N-Acetyl-chitohexaose

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)
Eluent       : CH3CN/H2O=70/30
Flow rate    : 1.0mL/min
Detector     : Shodex RI
Column temp. : 25deg-C