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Analysis of Saccharides and Organic Acids (RI and UV Detector)

The results of analysis of samples containing saccharides, organic acids and alcohols using SUGAR SH1821 are shown here. With RI detection, the peaks of these compounds overlap each other. However, with UV detection (at 210nm), there is a possibility of quantifying organic acids as saccharides have little absorbance at 210nm.

Sample :
1. Tartaric acid
2. Glucose
3. Malic acid
4. Fructose
5. Succinic acid
6. Glycerol
7. Acetic acid
8. Ethanol
9. Maleic acid
10. Fumaric acid

Columns      : Shodex SUGAR SH-G (6.0mmID*50mm) + SH1821 (8.0mmID*300mm)
Eluent       : 5mM H2SO4 aq.
Flow rate    : 1.0mL/min
Detector     : Shodex RI, UV(210nm)
Column temp. : 50deg-C