Cyclodextrin in Plasma

This is an example of a sensitive detection of cyclodextrin contained in plasma by pulse-amperometric electrochemical detector (PAD) using strong alkaline reagent mixed with eluent at post-column. Asahipak C8P-50 4E (a column for reversed phase chromatography) is used for the separation.


Sample : beta-Cyclodextrin
1. G-beta-CD
2. beta-CD

Columns      : Shodex Asahipak C8P-50 4E (4.6mmID*250mm)
Eluent       : 1mM Sodium hydroxide + 0.6% CH3CN
Reagent      : 1.5M Sodium hydroxide
Flow rate    : (Eluent); 0.6mL/min, (Reagent); 1.0mL/min 
Detector     : PAD
Column temp. : 50deg-C

Y.Kubota et al., Analytical Biochem., 201 (1992) 99.