Glucose

For substances, which are not chemiluminescent themselves, if they can be converted into hydrogen peroxide through an enzyme reaction, it is possible to make a chemiluminescence detection of them. For example, glucose itself cannot be detected chemiluminescently. However, by reacting glucose with glucose oxydase, gluconic acid and hydrogen peroxide are generated. Then, it is possible to make a sensitive detection of the hydrogen peroxide generated here by both direct and indirect emission type. This is an example of glucose measurement by using AFpak AGO-494, a column for immobilizing enzyme, and a chemiluminescence detector (CL).



Sample : 1. Glucose

Column       : Shodex AFpak AGO-494 (4.6mmID*10mm)
Eluent       : H2O
Reagent      : 5mM Luminol + 5micro-M Hemoglobin in 0.1M Phosphate-Na
Flow rate    : (Eluent); 0.2mL/min, (Reagent); 0.4mL/min 
Detector     : CL
Column temp. : 37deg-C