N-Acetylglucosamine and N-Acetylchitobiose

The ligand of AFpak AWG-894 column is wheat germ agglutinin (WGA) which has affinity with acetylglucosamine. The column is suited to high-selective analysis and fractionation for saccharides or glycoproteins.


Sample :
1. N-Acetylglucosamine
2. N-Acetylchitobiose

Column       : Shodex AFpak AWG-894 (8.0mmID*50mm)
Eluent       : 1/15M Phosphate buffer(pH7.4) + 0.15M NaCl
Flow rate    : 0.5mL/min
Detector     : UV(280nm)
Column temp. : Room temp.