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Organic Acids and Vitamin C in Fruit Juice

The main organic acids and Vitamin C contained in fruit are separated using RSpak KC-811 with 1mM perchloric acid as an eluent.


Sample : Fruit juice
1. Cl-
2. Oxalic
3. Maleic
4. Citric
5. Malic
6. Vitamin C
7. Succinic
8. Formic
9. Acetic

Columns      : Shodex RSpak KC-LG (8.0mmID*50mm) + KC-811 (8.0mmID*300mm) x 2
Eluent       : 1mM HClO4 aq.
Reagent      : 10-fold diluted ST3-R (for post-column method)
Flow rate    : (Eluent); 1.0mL/min, (Reagent); 0.4mL/min
Detector     : VIS(430nm)
Column temp. : 40deg-C