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Elution Volume of Organic Acids (NN-814)

Elution volume of organic acids using RSpak NN-814 is shown.

Substance Elution Volume
(mL)
Oxalic acid
7.6

Maleic acid

8.8
D-Glucuronic acid
8.9
Gluconic acid
9.0
alpha-Ketoglutaric acid
9.2
Tartaric acid
9.2
Pyruvic acid
9.4
Citric acid
9.5
Glyoxylic acid
9.7
Malic acid
9.8
Glycolic acid
9.8
Malonic acid
10.0
Pyroglutamic acid
10.0
Substance Elution v Volume
(mL)
Lactic acid
10.2
Formic acid
10.2
Citraconic acid
10.7
Succinic acid
10.8
Acetic acid
10.8
Levulinic acid
11.1
Glutaric acid
11.7
Itaconic acid
12.3
Adipic acid
12.9
Fumaric acid
13.4
trans-Crotonic acid
15.2
n-Capronic acid
30.8
L-Glutamic acid
37.8

Column       : Shodex RSpak NN-814 (8.0mmID*250mm)
Eluent       : 0.1% H3PO4 aq.
Column temp. : 60deg-C