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Elution Volume of Organic Acids (RSpak DE-413)

Elution volume of organic acids using RSpak DE-413 is shown.

Substance Elution Volume
(mL)
Oxalic acid
1.75
Glyoxylic acid
1.91
DL-Tartaric acid
2.17
Glycolic acid
2.22
beta-Hydroxypropionic acid
2.61
Formic acid
2.68
Malic acid
2.72
Pyruvic acid
2.74
Lactic acid
2.98
L-Pyroglutamic acid
3.07
Citric acid
3.12
Malonic acid
3.18
Substance Elution Volume
(mL)
Maleic acid
3.36
Acetic acid
3.37
alpha-Ketoglutaric acid
3.55
Succinic acid
4.70
Levulinic acid
6.64
Propionic acid
7.12
Itaconic acid
8.94
trans-Aconitic acid
10.80
Fumaric acid
11.09
Adipic acid
16.38
Mesaconic acid
28.48


Column      : Shodex RSpak DE-413 (4.6mmID*150mm)
Eluent      : 10mM H3PO4 aq.
Flow rate   : 1.0mL/min
Detector    : Shodex RI
Column temp.: 50deg-C