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Di-carboxylic Acids

The elution pattern of RSpak DE-413 and that of ODS column were compared. For di-carboxylic acids, the elution times of ODS column were faster than those of RSpak DE-413. To the contrary, for suberic acid whose carbon number of alkyl chain is six, the elution time of RSpak DE-413 is faster that of ODS column.

Sample : 5micro-L
1. Malonic acid (n=1) 0.7mg/mL
2. Succinic acid (n=2) 0.9mg/mL
3. Glutaric acid (n=3) 0.9mg/mL
4. Adipic acid (n=4) 1.2mg/mL
5. Pimelic acid (n=5) 2.0mg/mL
6. Suberic acid (n=6) 1.5mg/mL

Columns      : (above); Shodex RSpak DE-413 (4.6mmID*150mm)
               (below); ODS column from (other manfacturer)
Eluent       : 20mM H3PO4 aq./CH3CN=92/8
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : 40deg-C