Phosphorylated Saccharides (2)

Glucose can be anearobically decomposed into lactic acid and ethanol.This decomposing process is called as glucolytic pathway. In this pathway, some products such as glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, etc. are produced as intermediary products. Here, fructose-6-phosphate and fructose-1,6-bisphosphate are analyzed using Asahipak NH2P-50 4E by ion-exchange mode.


Sample : 5 mg/mL each, 20micro-L
1. Fructose-6-phosphate
2. Fructose-1,6-bisphosphate

Column       : Shodex Asahipak NH2P-50 4E (4.6mmID*250mm)				
Eluent       : 300mM Sodium phosphate buffer(pH4.4)						
Flow rate    : 1.0mL/min						
Detector     : Shodex RI						
Column temp. : 40deg-C