Separation of N-acetylneuraminic Acid and N-glycolyneuraminic Acid

N-acetylneuraminic acid and N-glycolyneuraminic acid, family of sialic acid, were separated using organic-acids-analysis column, RSpak KC-811.


Sample : 0.1% each, 40micro-L
1. N-Glycolylneuraminic acid
2. N-Acetylneuraminic acid

Column       : Shodex RSpak KC-811 (8.0mmID*300mm)
Eluent       : 1% H3P04 aq.
Flow rate    : 1.0mL/min
Detector     : UV(210nm)
Column temp. : 40deg-C