LC/MS Analysis of Drugs in BSA (2) (ODP2 HP)

This shows LC/MS analysis of drugs in BSA with a gradient using polymer-based ODP2 HP. Each drug has peak areas between 98 – 107 % (peak areas without BSA are set as 100 %), which means a good separation without receiving ion suppression from protein.


Sample : drugs 500ng/mL each in BSA (7.0mg/L), 5micro-L
Lidocaine
Chlorpheniramine
Propranolol
Diphenhydramine
Bupivacaine
Verapamil
Imipramine

Column       : Shodex ODP2 HP-2B (2.0mmID*50mm)
Eluent       : (A);10mM Ammonium acetate aq.
               (B);100mM Ammonium acetate aq./CH3CN=10/90
               gradient; (A) to (A) to 70%(B) to 70%(B) to (A),
			               0min to 5min to 10min to 15min to 15.01min
Flow rate    : 0.2mL/min
Detector     : ESI-MS (SIM Positive)
Column temp. : 40deg-C