Vitamin B₁

RSpak NN-614 is an improved version of OHpak M-414, which was recommended by a Japanese standard, "Methods of Analysis in Health Science" (2005). The total quantity of vitamin B₁ was measured using RSpak NN-614 with a phosphate buffer eluent. The vitamin B₁ separated by the column is converted into thiochrome using a postcolumn reagent and detected by a fluorescence detector.



Sample : Thiamine hydrochloride

Column       : Shodex RSpak NN-614 (6.0mmID*150mm)
Eluent       : 45mM KH2PO4 + 55mM Na2HPO4 aq.
Reagent      : 0.01% K3Fe(CN)6 + 15% NaOH
Flow rate    : (Eluent); 0.7mL/min, (Reagent); 0.7mL/min
Detector     : Fluorescence detector(Ex.: 375nm, Em.: 450nm)
Column temp. : 25deg-C