Margarine

Vitamin A palmitate was analyzed using GPC KF-801. Wave length of 325nm at which it has maximum UV absorption was used for the detection. Usually, saponification is necessary to analyze vitamin A as the sample pretreatment. However, in this analysis, no complicated sample pretreatment procedure was necessary. What is necessary is just to dissolve the sample in the eluent(THF) and filtrate it using a 0.45 micron disposable filter. This method using KF-801 is a very simple way to analyze vitamin A.


Sample : Margarine 0.2%, 100micro-L
1. Vitamin A palmitate

Columns      : Shodex GPC KF-801 (8.0mmID*300mm) x 2
Eluent       : THF
Flow rate    : 1.0mL/min
Detector     : UV(325nm)
Column temp. : 40deg-C