Catecholamines in Urine (ES-502C 7C)

Catecholamines in urine were analyzed using Asahipak ES-502C 7C (a column for weak cation exchange chromatography).


Sample : Catecholamines in Urine
E. Adrenaline
N. Norepinephrine
D. Dopamine

Column           : Shodex Asahipak ES-502C 7C (7.5mmID*100mm)
Eluent           : 0.05M Succinic acid + 0.15M sodium succinate + 0.5M EDTA(pH6.7)
Reaction reagent : (A); 0.1M glycylglycine containing 0.1M tartaric acid + 
                        0.1M boric acid + 2mM zinc sulphate(pH6.5)
                   (B); 0.25M potassium borate buffer(pH8.4) 
                        containing 0.01%(w/v) hexacyanoferrate(III)
Flow rate        : (Eluent) 1.0mL/min, (Reaction reagent) 0.35mL/min each				   
Detector         : Fruorescence (Ex 350nm, Em 500nm)
Column temp.     : 30deg-C
Reaction temp.   : 90deg-C

T. Seki et al., The Chemical Sciety of Japan, (7) (1986) 1040