Selection of Calibration Curves

A column, which has the most appropriated calibration curve for the sample molecular weight range should be selected. For example, regarding the two columns (1) and (2), whose calibration curves are shown in the figure, column (2) is best suited for the analysis of the mixture of A, B and C. In other words, among the column calibration curves which covers all the sample molecular weight range, a column whose calibration curve has the smallest inclination should be selected to obtain a good separation.