Comparison of Calibration Curves for Down-sized SEC Columns (HFIP Eluent: PMMA)

The calibration curves for downsized organic SEC (GPC) columns using PMMA are shown. In the case of the analysis for polymer whose MW is 1,000,000 or less, GPC LF-604 or LF-404 is suitable. When it is needed to separate low MW polymer and high MW polymer and low MW polymer is the purpose for the analysis, GPC HFIP-603 is suitable.
Please refer to Comparison of Analysis of PMMA with Down-sized SEC Columns



Sample : PMMA, Poly(methyl methacrylate) standards, 50micro-L

Columns      : Shodex GPC LF-604 (6.0mmID*150mm) x 2
               Shodex GPC LF-404 (4.6mmID*250mm) x 2
               Shodex GPC KF-405LHQ (4.6mmID*250mm) x 2
               Shodex GPC KF-406LHQ (4.6mmID*250mm) x 2
               Shodex GPC HFIP-603 (6.0mmID*150mm) x 2
               Shodex GPC HFIP-606M (6.0mmID*150mm) x 2    
Eluent       : 5mM CF3COONa in HFIP
Flow rate    : 0.3mL/min
Detector     : Shodex RI(small cell volume)
Column temp. : 40deg-C