Effect of Sample Load on Column Efficiency (1) (I-524A)

Anion standards were analyzed using IC I-524A. This shows the change in column efficiency with the concentration of the sample when the injection volume is 100micro-L. The column efficiency does not decrease until the sample concentration reaches 10mg/L for Cl^- and 100mg/L for SO₄^2-. Beyond those concentrations, HETP increases and peak becomes broad. When 1mg/L of Cl^- is injected, there is not decreasing in column efficiency until the injection volume reaches 500micro-L.


Sample :
Cl^-
SO₄^2-

HETP=(Column length)/(Theoretical plate number)

Column       : Shodex IC I-524A (4.6mmID*100mm)
Eluent       : 2.5mM Phthalic acid(pH4.0 adjusted by Tris(hydroxymethyl)aminomethane)
Flow rate    : 1.2mL/min
Detector     : Non-suppressed coductivity
Column temp. : 40deg-C