Effect of Acetonitorile Concentration on Elution Volume

Monovalent and divalent cation standards were analyzed using IC YK-421. The effect of the acetonitorile concentration in 3mM nitric acid on elution volume of cations is shown. By increasing the acetonitorile concentration, the cations are eluted faster. Especially, the elution volume of divalent cations are eluted faster markedly. When the acetonitorile concentration of acetonitrile is higher than 50%, to the contrary, cations are eluted slower a little by increasing acetonitorile concentration.


Sample :
Li⁺
Na⁺
NH₄⁺
K⁺
Mg²⁺
Ca²⁺

Column       : Shodex IC YK-421 (4.6mmID*125mm)
Eluent       : 3mM Nitric acid aq./CH3CN
Flow rate    : 1.0mL/min
Detector     : Non-suppressed coductivity
Column temp. : 40deg-C