Proteins in Normal Human Serum

Asahipak GS-520 7G, a multimode column, separates proteins by GFC mode. However, albumins such as bovine serumalbumin and human serum albumin are eluted slower because the albumins have affinity with the molecular structure of poly(vinylalcohol) gel which is packed in GS-520. Therefore, albumins can separate with components of similar molecular weight. Here, albumin (ca, MW 69,000) is separated with tranferrin (ca. MW 80,000).
GS-520 7G is an old type of GS-520 HQ.


Sample :
1. IgM
2. IgG
3. Transferrin
4. Mercaptoalbumin
5. Nonmercaptoalbumin
6. Creatinine
7. Uric acid

Columns      : Shodex Asahipak GS-520 7G (7.5mmID*500mm) x 4
Eluent       : 0.03M Sodium phosphate buffer(pH7.0) + 0.15M Na2SO4
Flow rate    : 1.0mL/min
Detector     : UV(250nm)
Column temp. : 30deg-C