Mercaptpalbumin and Nonmercaptoalbumin by Ion Exchange Chromatography

Mercaptpalbumin and nonmercaptoalbumin were analyzed using Asahipak ES-502N (a column for weak anion exchange chromatography). The ratio of human mercaptoalbumin (HMA) to human nonmercaptoalbumin (HNA) in serum is thought to have some relation with aging. The correlation between the ratio and various types of illness is being studied.


Sample :
Globulin
Uric acid
HMA, Human Mercaptoalbumin
HNA, Human Nonmercaptoalbumin

Column       : Shodex Asahipak ES-502N 7C (7.5mmID*100mm)
Eluent       : 50mM N-methylpiperazine-HCl buffer(pH4.8) + 400mM Na2SO4 + 0.3% C2H5OH
Flow rate    : 1.0mL/min
Detector     : UV(280nm)
Column temp. : 35deg-C