Analysis of Gelatin by PAGI Method (2)

Gelatin contains alpha, beta and gamma chains, and its molecular weight distribution has a significant influence on the characteristics of photographic films and on the crystallization of silver halides in an emulsion. Asahipak GS-620 7G-P is especially suitable for analysis of the components of gelatin and the combination of OHpak SB-805 HQ and SB-804 HQ is suitable for molecular weight distribution analysis by the PAGI method (test method of gelatin for photo film in Japan).


Sample : Gelatin (Koepff 16922) 0.2%
100micro-L

Columns      : Shodex Asahipak GS-620 7G-P (7.5mmID*500mm) x 2
               Shodex OHpak SB-805 HQ x 3 + SB-804 HQ (8.0mmID*300mm each)
Eluent       : 0.1M KH2PO4 aq./0.1M Na2HPO4 aq.=50/50
Flow rate    : 1.0mL/min
Detector     : UV(230nm)
Column temp. : 50deg-C