Features of RSpak DE Series (6) (Effect of Flow Rate on Column Efficiency)

When di-n-butyl ketone is analyzed using RSpak DE-413 and DE-413L with water/acetonitrile eluent, a decease of theoretical plate number was observed according to an increase of flow rate. The decrease was marked when the flow rate was faster than 1mL/min.



Sample :
(Right); Di-n-butyl ketone
(Center, Left);
1. 0.1% Methylethyl ketone
2. 0.1% Diethyl ketone
3. 0.2% Di-n-propyl ketone
4. 0.3% Di-n-butyl ketone

Columns      : Shodex RSpak DE-413 (4.6mmID*150mm), DE-413L (4.6mmID*250mm)
Eluent       : CH3CN/H2O=50/50
Detector     : UV(262nm)
Column temp. : 40deg-C