Feature of High Temperature Analysis (1) (High Resolution)
ET-RP1 4D is polymer base column for high temperature analysis.
Figure shows comparison of reduced plate height and analysis time between 40deg-C and 150deg-C.
Smaller value of reduced plate height means better column resolution. The reduced plate height
of acetophenone and benzene decreased at the 150deg-C.
Mobile phase viscosity decreases at higher temperature and thus enhanced diffusion rate
between the eluent and the polymeric stationary phase. Then resolution is improved.
Sample :
1.
Uracil
2.
Pyridine
3.
Acetophenone
4.
Benzene
(notes) Eluent was introduced to column after preheating and introduced to detector after cooling.
| Column |
: Shodex ET-RP1 4D (4.6mmID*150mm) |
: Shodex ET-RP1 4D (4.6mmID*150mm) |
| Eluent |
: H2O/CH3CN=50/50 |
: H2O/CH3CN=75/25 |
| Flow rate |
: 0.5mL/min |
: 2.4mL/min |
| Detector |
: Diode array (210nm) |
: Diode array (210nm) |
| Column temp. |
: 40deg-C |
: 150deg-C |
*Column oven : Polaratherm 9000 Series (SandraSelerity Technologies, Inc)
Coutesy of Research Institude for Chromatography bvba