Oligopeptides (1)
Oligopeptides were analyzed using Asahipak
ODP-50 6D (a column for reversed phase chromatography).
Sample :
1.
Val-Glu-Glu-Ala-Glu
2.
Gly-Gly-Phe
3.
Met-Enkephalin
4.
Bradykinin
5.
Angiotensin II
6.
Angiotensin III
7.
Phe-Phe-Phe
Column : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent : (1): (A); 0.1M NaClO4 + 0.1% Sodium phosphate(pH2)
(B); CH3CN
Linear gradient: 0min to 60min, (A)/(B)=90/10 to 60/40
(2): (A); 0.1M NaClO4 + 0.1% Sodium phosphate(pH9)
(B); CH3CN
Linear gradient: 0min to 60min, (A)/(B)=90/10 to 60/40
Flow rate : 1.0mL/min
Detector : UV(220nm)
Column temp. : 30deg-C