Opioid Peptides (1) (By Post-column Reaction Method)

Opioid peptides were analyzed using Asahipak ODP-50 6D (a column for reversed phase chromatography) by post-column reaction method.


Sample :
1. Tyr-Gly
2. Tyr-Gly-Gly
3. Tyr-Gly-Gly-Phe
4. Tyr-Phe
5. ME-Thr-ser-Glu-Lys
6. ME, Met-Enkephalin
7. LE, Leu-Enkephalin
8. ME-Arg, Met-Enkephalin-Arg
9. alpha-EDP, alpha-Endorphin
10. LE-Arg, Leu-Enkephalin-Arg
11. ME-NH₂, Met-Enkephalin-NH₂
12. gamma-EDP, alpha-EDP-Leu
13. ME-Arg-Glu-Leu
14. LE-NH₂, Leu-Enkephalin-NH₂
15. ME-Arg-Phe
16. DNP 1-8

Column       : Shodex Asahipak ODP-50 6D (6.0mmID*150mm)
Eluent       : (A); 50mM Sodium borate(pH10.0)/H2O/CH3CN=4/15/1
               (B); 50mM Sodium borate(pH10.0)/H2O/CH3CN=1/1/3
               Gradient: (A) to (B)
Flow rate    : 1.0mL/min
Detector     : Fluorescnce(Ex. 330nm, Em. 430nm)
Column temp. : Room temp.

Guo-Qing Zhang et al., Analytical Science, 6 (1990) 671.