Recovery of Proteins and Enzime Activity (QA-825)

Recoveries of proteins and enzime activity for IEC QA-825 (a column forstrong anion exchange chromatography) are shown. When 0.5mg of each proteins was injected into the column by linear gradient elution, in which salt concentration increases linearly over one minute, sample recovery was found to be good. Then the same amount of enzyme was injected into the column in the same manner as the above protein and its recovery was also found to be good.

Recovery of proteins
Protein	Recovery (%)
Ovalbumin	106
Conalbumin	100
beta- Lactoglobulin	99
Trypsin inhibitor	99

Recovery of enzyme activity
Enzyme	Recovery (%)
Kallikrein	95
Lipoxidase	100
Catalase	96

Sample load : 0.5mg each

Column : Shodex IEC QA-825 (8.0mmID*75mm)
Eluent : (A); 20mM Tris-HCl buffer(pH7.5)
         (B); (A) + 0.5M NaCl
         Linear gradient: (A) to (B), 1min