Standard Proteins (8) (ES-502C 7C)
Four kinds of protien standard were analyzed using Asahipak
ES-502C 7C (a column for weak cation exchange chromatography).
Sample :
1.
Myoglobin
2.
Ribonuclease A
3.
Chymotrypsinogen A
4.
Lysozyme
Column : Shodex Asahipak ES-502C 7C (7.5mmID*100mm)
Eluent : (A); 50mM Sodium phosphate buffer(pH7.0)
(B); (A) + 0.5M NaCl
Linear gradient: 0min to 20min, (A) to (B)
Flow rate : 1.0mL/min
Detector : UV(280nm)