Column : Shodex IEC QA-825 (8.0mmID*75mm) Eluent : (A); 20mM Tris-HCl buffer(pH8.5) (B); (A) + 0.5M NaCl Linear gradient: 0min to 60min, (A) to (B) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : 25deg-C