Standard Proteins (20) (SP-420N)

Five kinds of protein standards were analyzed using IEC SP-420N ( a column for strong cation exchange chromatography packed with non-porous gel ).


Sample :
1. Ovalbumin
2. Trypsinogen
3. Ribonuclease A
4. Cytochrome c
5. Lysozyme

Column       : Shodex IEC SP-420N (4.6mmID*35mm)
Eluent       : (A); 20mM MES* buffer(pH6.0)
               (B); (A) + 0.5M Na2SO4
               Linear gradient: 0min to 15min, (A) to (B)
Flow rate    : 1.5mL/min
Detector     : UV(280nm)
Column temp. : Room temp.

*MES: 2-(N-Morpholino)ethanesulfonic acid