Standard Proteins (10) (DEAE-825, DEAE3N-4T)

Three kinds of protein standards were analyzed using IEC DEAE3N-4T (a column for weak anion exchange chromatography packed with non-porous gel). Fast analysis is possible using DEAE-420N compared with using IEC DEAE-825 (a column for weak anion exchange chromatography packed with porous gel).


Sample :
1. Conalbumin 0.4mg/mL
2. Ovalbumin 1.2mg/mL
3. Trypsin inhibitor 1.0mg/mL

Columns      : Shodex IEC DEAE-825 (8.0mmID*75mm)          : Shodex IEC DEAE3N-4T (4.6mmID*35mm)
Eluent       : (A); 20mM Piperazine-HCl buffer(pH6.0)      : (A); 25mM Piperazine-HCl buffer(pH6.0)
               (B); (A) + 0.5M NaCl                          (B); (A) + 0.5M NaCl
               Linear gradient: 0min to 60min, (A) to (B)    Linear gradient: 0min to 10min, (A) to (B)
Flow rate    : 1.0mL/min                                   : 1.5mL/min
Detector     : UV(280nm)                     
Column temp. : Room temp.