Recovery of Proteins and Enzyme Activity (PH-814)

Recovery of proteins and enzyme activity were measured using HIC PH-814 ( a hydrophobic interaction column ). With the exception of myoglobin, which had a recovery of 93%, all proteins show good recovery values. Enzyme activity for all proteins remained high, except for catalase which had a value of 88% and hexokinase which had a value of 94%.

Recovery of proteins
Protein	Recovery(%)
Cytochrome c	104
Myoglobin	93
Ribonuclease A	100
Ovalbumin	103
Lysozyme	100
alpha Chymotrypsin	99
alpha Chymotrypsinogen A	96

Recovery of enzyme activity
Enzyme	Recovery(%)
Trypsin	96
Hexokinase	94
Catalase	88
Lysozyme	100
Lipoxidase	100

Sample load : 0.5mg each

Column : Shodex HIC PH-814