Column : Shodex AFpak ADS-894 (8.0mmID*50mm) Eluent : (A); 0.02M Tris-HCl(pH7.4) (B); (A) + 1.0M NaCl Linear gradient: (A) to (B) for 60min Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : Room temp.