Column : Shodex AFpak ACB-894 (8.0mmID*50mm) Eluent : (A); 0.1M Potassium phosphate buffer(pH5.0) (B); 0.1M Potassium phosphate buffer(pH7.5) + 1.5M KCl Step gradient: (A) to (B) Flow rate : 1.0mL/min Detector : UV(280nm) Column temp. : Room temp.