Column : Shodex AFpak AIA-894 (8.0mmID*50mm) Eluent : (A); 0.1M Sodium acetate buffer(pH7.7) + 0.5M NaCl (B); 0.05M Tris-HCl(pH8.0) + 0.15M NH4Cl Step gradient: (A) to (B) Flow rate : 0.5mL/min Detector : UV(280nm) Column temp. : Room temp.